Biologist (Genomics) (50865)
Position Objective: The National Institutes of Health requires contractor support for laboratory and computational analysis of genomic and epigenomic data. The work supports ongoing research involving genotyping, long-range sequencing, and DNA methylation profiling at the National Institute on Aging (NIA) within the National Institutes of Health (NIH).
Duties and Responsibilities:
- Evaluate: Read quality, coverage, and depth and Sample integrity and contamination
- Flag anomalies and provide preliminary interpretation.
- Perform DNA extraction from blood, tissue, or cultured cells using established protocols. 1
- Conduct genotyping assays (e.g., SNP arrays, PCR-based methods 2
- Support long-range sequencing workflows (e.g., library prep and sequencing using long-read platforms such as PacBio or Oxford Nanopore). 3
- Perform DNA methylation assays (e.g., bisulfite conversion, array-based or sequencing-based methods).
- Execute established QC pipelines for sequencing and genotyping data.
- Compile and document QC metrics in standardized formats.
- Modify existing scripts and pipelines to support project needs.
- Perform data analysis using: R (statistical analysis and visualization) and Scripting (Python, Bash, or equivalent)
- Conduct: Variant calling and basic annotation and DNA methylation data processing and analysis and Data integration across datasets when directed
- Maintain accurate laboratory notebooks and electronic records.
- Identify routine technical issues and implement standard troubleshooting steps.
- Prepare: Summary tables and figures and Written summaries of methods and results
- Present findings in internal meetings to NIH staff
- Contribute to draft reports, manuscripts, or project updates.
- Sample Processing Logs DNA extraction records and yields
- Genotyping/Sequencing Outputs Raw and processed data files
- QC Reports Standardized quality metrics and summaries
- Analysis Outputs Variant and methylation results, visualizations
- Scripts and Code Documented R/Python/Bash scripts
- Monthly Summary of work performed and findings
- Presentation Materials Slides or briefings for NIH staff