Scientist I

Responsibilities :
The Scientist I will be a vital contributor to our non-viral cell and gene therapy delivery platform, primarily focused on R&D for innovative nucleic acid payload technologies. This role involves evaluating novel mRNA and DNA designs using a range of molecular biology techniques, including plasmid cloning, in vitro validation, and the production of in vitro-transcribed mRNA. To verify successful expression, the scientist will utilize assays such as Western blot, qRT-PCR, and luciferase reporter assays.

• Design and clone expression vectors and IVT donor plasmids to support research initiatives.
• Execute DNA and mRNA transfections in mouse and human cell lines.
• Assess nucleic acid expression through molecular methods like qPCR, reporter assays and Western blotting.
• Maintenance of detailed electronic laboratory notebooks (ELN) and preparation of summary slides

Required Skills:

• Education/Experience: Bachelor's degree with 2+ years or a Master's degree with 1+ years of experience in molecular biology, pharmaceutical sciences, or a related field.
• Molecular Biology: Required proficiency in advanced cloning (Gibson assembly, Golden Gate, site-directed mutagenesis) and IVT.
• Functional Assays: Hands-on expertise in measuring transgene delivery success via reporter assays and qRT-PCR.
• Communication: Exceptional oral and written skills with a meticulous attention to detail.
• Must haves for this position are:

• Molecular cloning and DNA manipulation techniques (e.g. Gibson assembly, GoldenGate cloning, site-directed mutagenesis)
• Work with RNA, especially preparing in vitro-transcribed mRNA
• Experience with molecular assays to assess gene - e.g. Western blot, luciferase reporter assays, qRT-PCR)

Preferred Skills:

• Cell Culture: Preferred experience in genetic manipulation of cell lines and primary cells (e.g., T cells, monocytes, hepatocytes).
• Software/Tools: Desired proficiency in Snapgene, Prism, and MS Office.